血管生成抑制剂SU6668对SCID小鼠胃癌生长和转移的影响

[Effect of angiogenesis inhibitor SU6668 on the growth and metastasis of gastric cancer in SCID mice].

作者信息

Jiang Xiao-ting, Tao Hou-quan, Zou Shou-chun

机构信息

Clinical Laboratory Medical Center, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2006 Jul;9(4):335-7.

PMID:16886118

Abstract

OBJECTIVE

To study the effect of angiogenesis inhibitor SU6668 on the growth and metastasis of gastric cancer in SCID mice.

METHODS

Metastatic model was established by orthotopic implantation of histologically intact human tumor tissue into the gastric wall of SCID mice. Forty-eight mice were randomly divided into four groups, and saline, 5-FU, SU6668, and 5-FU plus SU6668 were administered by i.p. every day for 6 weeks after tumor implantation. The mice were killed and tumor weight, tumor inhibition rate, intratumoral microvessel density(MVD), apoptotic index(AI) and metastasis inhibition were evaluated.

RESULTS

Compared with the control, tumor growth was significantly inhibited in mice treated respectively with 5-FU, SU6668 and 5-FU plus SU6668 with inhibition rates of 47.5%, 64.1% and 69.2% respectively. Decreased MVD and increased AI were noted in the mice treated with SU6668 and 5-FU plus SU6668. The incidences of liver and peritoneal metastases was significantly inhibited and decreased to 62.5%, 69.9% in SU6668 group, and 74.9%, 90% in 5-FU plus SU6668 group. The growth and metastasis of human gastric cancer implanted in SCID mice were significantly inhibited in SU6668 group and combined group, especially in combined group.

CONCLUSION

Angiogenesis inhibitor SU6668 has a strong inhibitory effect on tumor growth and metastasis of human gastric cancer transplanted in SCID mice, and has synergistic effect combined with cytotoxic agents.

摘要

目的

研究血管生成抑制剂SU6668对SCID小鼠胃癌生长和转移的影响。

方法

通过将组织学完整的人肿瘤组织原位植入SCID小鼠胃壁建立转移模型。48只小鼠随机分为四组，肿瘤植入后每天腹腔注射生理盐水、5-氟尿嘧啶、SU6668以及5-氟尿嘧啶加SU6668，持续6周。处死小鼠后评估肿瘤重量、肿瘤抑制率、瘤内微血管密度（MVD）、凋亡指数（AI）和转移抑制情况。

结果

与对照组相比，分别用5-氟尿嘧啶、SU6668以及5-氟尿嘧啶加SU6668处理的小鼠肿瘤生长明显受到抑制，抑制率分别为47.5%、64.1%和69.2%。SU6668以及5-氟尿嘧啶加SU6668处理的小鼠MVD降低，AI升高。肝转移和腹膜转移的发生率明显受到抑制，SU6668组分别降至62.5%、69.9%，5-氟尿嘧啶加SU6668组分别降至74.9%、90%。SU6668组和联合组中植入SCID小鼠的人胃癌的生长和转移明显受到抑制，尤其是联合组。