Trifiletti R, Hyman B, LaVia M, Knapp W, Virella G
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston 29425.
Scand J Immunol. 1990 Jan;31(1):25-31. doi: 10.1111/j.1365-3083.1990.tb02739.x.
In this report we compare the effect of stimulation of peripheral mononuclear cells (PBMC) by using two monoclonal antibodies (MoAb) directed against the CD2 receptor on T cells or by using autologous erythrocytes (E) which express on their surface lymphocyte function-associated antigen 3 (LFA3), a natural ligand for CD2. The addition of autologous erythrocytes to pokeweed mitogen (PWM)-stimulated PBMC results in the enhancement of polyclonal immunoglobulin synthesis and of antigen-specific B-cell responses. Because B cells lack the CD2 molecule, it can be concluded that their enhanced activity is a consequence of the delivery of activating signals by activated T lymphocytes. When PBMC cultures were stimulated with a pair of anti-CD2 MoAb (Leu5b and VIT13) we were able to induce polyclonal immunoglobulin synthesis, particularly IgM, in cultures supplemented with interleukin 2(IL-2). Specific responses to tetanus toxoid (TT) and keyhole limpet haemocyanin (KLH) were also enhanced by the addition of autologous E to PWM-stimulated PBMC. Significant anti-TT responses were observed in cultures stimulated with E + TT + IL-2. In contrast, stimulation of PBMC with VIT13 + Leu5b + IL-2 + antigen was not effective in inducing anti-TT antibody and only weakly effective in inducing anti-KLH antibodies. Replacing Leu5b by anti-CD3 had no effect on the induction of specific antibody responses; in contrast, replacement of Leu5b by E enhanced anti-TT antibody production while the effect on polyclonal production of IgM was minimal. Therefore, it appears that the signal delivered by the association of CD2 with LFA3 is a better potentiating signal for specific B-cell responses than the signal delivered by pairs of MoAb to different epitopes of CD2 or to CD2 and CD3 epitopes.
在本报告中，我们比较了使用两种针对T细胞CD2受体的单克隆抗体（MoAb）刺激外周血单个核细胞（PBMC），或使用在其表面表达淋巴细胞功能相关抗原3（LFA3，CD2的天然配体）的自体红细胞（E）刺激PBMC的效果。将自体红细胞添加到美洲商陆有丝分裂原（PWM）刺激的PBMC中，可增强多克隆免疫球蛋白的合成以及抗原特异性B细胞反应。由于B细胞缺乏CD2分子，因此可以得出结论，它们活性的增强是活化T淋巴细胞传递激活信号的结果。当用一对抗CD2 MoAb（Leu5b和VIT13）刺激PBMC培养物时，我们能够在添加白细胞介素2（IL-2）的培养物中诱导多克隆免疫球蛋白的合成，尤其是IgM。向PWM刺激的PBMC中添加自体E也增强了对破伤风类毒素（TT）和钥孔戚血蓝蛋白（KLH）的特异性反应。在用E + TT + IL-2刺激的培养物中观察到了显著的抗TT反应。相比之下，用VIT13 + Leu5b + IL-2 +抗原刺激PBMC在诱导抗TT抗体方面无效，而在诱导抗KLH抗体方面效果微弱。用抗CD3替代Leu5b对特异性抗体反应的诱导没有影响；相反，用E替代Leu5b增强了抗TT抗体的产生，而对IgM多克隆产生的影响最小。因此，似乎CD2与LFA3结合传递的信号比MoAb对CD2不同表位或对CD2和CD3表位传递的信号更能有效增强特异性B细胞反应。
