睾丸酮丛毛单胞菌TA441的ORF18缺失突变体在与类固醇孵育后会积累大量的9,17-二氧代-1,2,3,4,10,19-六降雄甾烷-5-羧酸及其衍生物。
ORF18-disrupted mutant of Comamonas testosteroni TA441 accumulates significant amounts of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and its derivatives after incubation with steroids.
作者信息
Horinouchi Masae, Hayashi Toshiaki, Koshino Hiroyuki, Kudo Toshiaki
机构信息
RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
出版信息
J Steroid Biochem Mol Biol. 2006 Sep;101(1):78-84. doi: 10.1016/j.jsbmb.2006.06.006. Epub 2006 Aug 7.
In a steroid degradation gene cluster of Comamonas testosteroni TA441 consisting of ORF18, 17 and tesIHA2A1DEFG, ORF18 was implicated in encoding a CoA-transferase by database searches, but the matching substrate was not clear. In this study, ORF18 was shown to be necessary for conversion of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, a product of hydrolysis of 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-dien-4-oic acid in steroid degradation by TA441. The ORF18-disrupted mutant accumulates 7-hydroxy-9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and 7,12-dihydroxy-9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid when incubated with chenodeoxycholic acid and cholic acid, respectively.
在睾丸酮丛毛单胞菌TA441的一个甾体降解基因簇中，该基因簇由开放阅读框（ORF）18、17和tesIHA2A1DEFG组成，通过数据库搜索表明ORF18参与编码一种辅酶A转移酶，但匹配的底物尚不清楚。在本研究中，结果表明ORF18对于TA441在甾体降解过程中4,5-9,10-断-3-羟基-5,9,17-三氧代雄甾-1(10),2-二烯-4-酸水解产物9,17-二氧代-1,2,3,4,10,19-六降雄甾-5-酸的转化是必需的。当分别与鹅去氧胆酸和胆酸一起温育时，ORF18缺失突变体积累7-羟基-9,17-二氧代-1,2,3,4,10,19-六降雄甾-5-酸和7,12-二羟基-9,17-二氧代-1,2,3,4,10,19-六降雄甾-5-酸。
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