Liras A, Argomaniz L, Llorente P
Departmento de Bioquímica, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Biochim Biophys Acta. 1990 Jan 29;1033(1):114-7. doi: 10.1016/0304-4165(90)90203-9.
5'-Phosphoribosylpyrophosphate amidotransferase, which catalyzes the synthesis of phosphoribosylamine in the de novo synthesis of purine nucleotides, has been detected and partially purified approx. 800-fold from Artemia sp. nauplii. The apparent Km values for 5'-phosphoribosyl 1-pyrophosphate as substrate were 0.7 mM and 0.4 mM in the presence of glutamine and ammonia as nitrogenous sources, respectively, and the enzymatic activity was inhibited by purine 5'-ribonucleotide compounds and 5', 5'''-p1, p4-diguanosine tetraphosphate.
5'-磷酸核糖焦磷酸酰胺转移酶催化嘌呤核苷酸从头合成中磷酸核糖胺的合成，已从卤虫无节幼体中检测到并部分纯化了约800倍。以5'-磷酸核糖1-焦磷酸为底物时，在谷氨酰胺和氨作为氮源存在下，其表观Km值分别为0.7 mM和0.4 mM，并且该酶活性受到嘌呤5'-核糖核苷酸化合物和5', 5'''-p1, p4-二鸟苷四磷酸的抑制。
