Juárez-Méndez Sergio, Carretero Rosalba, Martínez-Tellez Rubelia, Silva-Gómez Adriana B, Flores Gonzalo
Escuela de Biología, Universidad Autónoma de Puebla, Puebla, México.
Synapse. 2006 Nov;60(6):450-5. doi: 10.1002/syn.20318.
We studied the morphological changes of the dendritic length of the pyramidal neurons of the prefrontal cortex (PFC) induced by the effect of chronic administration of caffeine in the neonatal rat. The caffeine (50 mg/kg, s.c.) was injected from day 1 after birth (P1) to day 12 (P12). The morphology of the pyramidal neurons of layer 3 of the PFC was investigated in these animals at two different ages, before puberty (P35) and after puberty (P70). Before the animals were sacrificed by using overdoses of sodium pentobarbital and being perfused intracardially with 0.9% saline, the locomotor activity in a novel environment was measured. The brains were then removed, processed by the Golgi-Cox stain, and analyzed by the Sholl method. The dendritic morphology clearly showed that the neonatal animals administered caffeine showed an increase in the dendritic length of the pyramidal neurons of the PFC when compared with the control animals at both ages. The present results suggest that neonatal administration of caffeine may in part affect the dendritic morphology of the pyramidal cells of this limbic structure and this effect persists after puberty and may be implicated in several brain processes.
我们研究了新生大鼠长期给予咖啡因后，前额叶皮质（PFC）锥体细胞树突长度的形态学变化。从出生后第1天（P1）至第12天（P12）皮下注射咖啡因（50 mg/kg）。在青春期前（P35）和青春期后（P70）这两个不同年龄段，对这些动物的PFC第3层锥体细胞的形态进行了研究。在用过量戊巴比妥钠处死动物并经心脏灌注0.9%生理盐水之前，测量其在新环境中的运动活性。然后取出大脑，用高尔基-考克斯染色法处理，并采用肖尔方法进行分析。树突形态学清楚地表明，与两个年龄段的对照动物相比，给予咖啡因的新生动物PFC锥体细胞的树突长度增加。目前的结果表明，新生期给予咖啡因可能部分影响该边缘结构锥体细胞的树突形态，且这种影响在青春期后持续存在，并可能与多个脑过程有关。
