用于测定生物缓冲液中组胺和1-甲基组胺的高效液相色谱法。
High-performance liquid chromatographic method for the determination of histamine and 1-methylhistamine in biological buffers.
作者信息
von Vietinghoff Vivica, Gäbel Gotthold, Aschenbach Jörg R
机构信息
Institute for Veterinary Physiology, University of Leipzig, An den Tierkliniken 7, D-04103 Leipzig, Germany.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Dec 5;844(2):335-9. doi: 10.1016/j.jchromb.2006.07.017. Epub 2006 Aug 8.
A method for the determination of histamine and its catabolite 1-methylhistamine (1-MH) was developed, using HPLC with fluorescence detection. Derivatization of both compounds occurred on-column with o-phthaldialdehyde dissolved in an alkaline borate buffer, followed by separation on a reversed phase C18 column. Histamine and 1-MH could be detected with comparable sensitivity (limit of quantification, 50 nM). The method was proven suitable to investigate catabolism of histamine by epithelia of pig colon. The method should be useful in research on histamine metabolism.
开发了一种使用带荧光检测的高效液相色谱法测定组胺及其分解代谢物一甲基组胺（1-MH）的方法。两种化合物均在柱上与溶解于碱性硼酸盐缓冲液中的邻苯二甲醛发生衍生化反应，随后在反相C18柱上进行分离。组胺和1-MH能够以相当的灵敏度检测到（定量限为50 nM）。该方法经证实适用于研究猪结肠上皮细胞对组胺的分解代谢。该方法在组胺代谢研究中应会有用。
Zotero 插件