用于蛋白激酶C选择性检测的合成肽底物。

A synthetic peptide substrate for selective assay of protein kinase C.

作者信息

Yasuda I, Kishimoto A, Tanaka S, Tominaga M, Sakurai A, Nishizuka Y

机构信息

Department of Biochemistry, Kobe University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Feb 14;166(3):1220-7. doi: 10.1016/0006-291x(90)90996-z.

DOI:10.1016/0006-291x(90)90996-z

PMID:1689574

Abstract

Among various phosphate acceptor proteins and peptides so far tested, a synthetic peptide having the sequence surrounding Ser(8) of myelin basic protein, Gln-Lys-Arg-Pro-Ser(8)-Gln-Arg-Ser-Lys-Tyr-Leu, (MBP4-14), is the most specific and convenient substrate which can be used for selective assay of protein kinase C. This peptide is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca2+/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts. The Km value is considerably low (7 microM) with a Vmax value of twice as much as that for H1 histone.

摘要

在目前已测试的各种磷酸受体蛋白和肽中，一种具有髓鞘碱性蛋白Ser(8)周围序列的合成肽，即Gln-Lys-Arg-Pro-Ser(8)-Gln-Arg-Ser-Lys-Tyr-Leu（MBP4-14），是最特异且方便的底物，可用于蛋白激酶C的选择性测定。该肽不会被环磷酸腺苷依赖性蛋白激酶、酪蛋白激酶I和II、Ca2+/钙调蛋白依赖性蛋白激酶II或磷酸化酶激酶磷酸化，并且可常规用于在粗组织提取物中以低背景测定蛋白激酶C。其Km值相当低（7 microM），Vmax值是H1组蛋白的两倍。