磷酸铝对聚氨酯泡沫固定化蓝藻碱性磷酸酶活性的影响。

Effect of aluminum phosphate on alkaline phosphatase activity of polyurethane foam immobilized cyanobacteria.

作者信息

Ramalingam N, Prasanna B Gowtham

机构信息

Tamil Nadu Rice Research Institute, Tamil Nadu Agricultural University, Aduthurai, Tanjore, India.

出版信息

Curr Microbiol. 2006 Sep;53(3):194-7. doi: 10.1007/s00284-005-0443-x. Epub 2006 Jul 28.

DOI:10.1007/s00284-005-0443-x

PMID:16897258

Abstract

The impact of insoluble phosphorus such as aluminum and rock phosphate on alkaline phosphatase activity of polyurethane foam immobilized cyanobacteria was assessed. Polyurethane foam immobilized Nodularia recorded the highest alkaline phosphatase activity of 9.04 (m. mol p-nitrophenol released h(-1) mg(-1) protein) in vitro. A higher concentration of aluminum phosphate was recorded a 25% reduction in alkaline phosphatase activity, ammonia content, and available phosphorus in culture filtrate of polyurethane foam immobilized cyanobacteria. In general, immobilized cyanobacteria exhibited a higher alkaline phosphatase activity in rock phosphate than aluminum phosphate.

摘要

评估了诸如铝和磷矿石等不溶性磷对聚氨酯泡沫固定化蓝藻碱性磷酸酶活性的影响。聚氨酯泡沫固定化的念珠藻在体外记录到最高碱性磷酸酶活性为9.04（毫摩尔对硝基苯酚释放 h⁻¹毫克⁻¹蛋白质）。较高浓度的磷酸铝使聚氨酯泡沫固定化蓝藻培养滤液中的碱性磷酸酶活性、氨含量和有效磷降低了25%。总体而言，固定化蓝藻在磷矿石中的碱性磷酸酶活性高于在磷酸铝中的活性。