新普拉瑟，一种来自日本新叶藻IFB-E016的新型具有细胞毒性和抗真菌作用的内生菌代谢产物。

Neoplaether, a new cytotoxic and antifungal endophyte metabolite from Neoplaconema napellum IFB-E016.

作者信息

Wang Feng W, Ye Yong H, Chen Jing R, Wang Xiao T, Zhu Hai L, Song Yong C, Tan Ren X

机构信息

Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, China.

出版信息

FEMS Microbiol Lett. 2006 Aug;261(2):218-23. doi: 10.1111/j.1574-6968.2006.00358.x.

DOI:10.1111/j.1574-6968.2006.00358.x

PMID:16907723

Abstract

A new diphenyl ether, named neoplaether, together with five known compounds monomethylsulochrin, physcion, helvolic acid, ergosterol and ergosterol peroxide, was isolated from the culture of Neoplaconema napellum IFB-E016, an endophytic fungus residing in the healthy leaves of Hopea hainanensis. The structure of neoplaether was elucidated by a correlative interpretation of its infrared spectrometry, mass spectroscopy and nuclear magnetic resonance spectra, and confirmed by its single-crystal X-ray diffraction analysis. Neoplaether exhibited significant cytotoxic activity against the human nasopharyngeal epidermoid tumor KB cell line, with an IC(50) value of 13.0 microg mL(-1), comparable to that of 5-fluorouracil (2.5 microg mL(-1)) co-assayed as a positive reference. In addition, it showed antifungal activity against Candida albicans, with a minimal inhibitory concentration value of 6.2 microg mL(-1) (amphotericin as a positive control had a value of 1.5 microg mL(-1)).

摘要

从海南坡垒健康叶片中的内生真菌椭圆新拟层孔菌（Neoplaconema napellum）IFB-E016的培养物中，分离出一种名为新普拉醚的新型二苯醚，以及5种已知化合物，即单甲基苏罗克菌素、大黄素甲醚、赫沃利酸、麦角甾醇和过氧化麦角甾醇。通过对其红外光谱、质谱和核磁共振光谱的相关解释阐明了新普拉醚的结构，并通过单晶X射线衍射分析得到证实。新普拉醚对人鼻咽表皮样肿瘤KB细胞系表现出显著的细胞毒活性，IC50值为13.0 μg mL-1，与作为阳性对照共同检测的5-氟尿嘧啶（2.5 μg mL-1）相当。此外，它对白色念珠菌显示出抗真菌活性，最小抑菌浓度值为6.2 μg mL-1（两性霉素作为阳性对照的值为1.5 μg mL-1）。