Reed Steven I
Department of Molecular Biology, MB-7, The Scripps Research Institute, La Jolla, California 92037, USA.
Cell Cycle. 2006 Sep;5(17):1923-4. doi: 10.4161/cc.5.17.3198. Epub 2006 Sep 1.
The recent description of a role for an SCF ubiquitin ligase in prolyl isomerization rather than ubiquitin ligation has led to a 2-step model for inactivation of cyclin E and, possibly, other SCF(Cdc4) targets in mammalian cells. In this proposed model, targets of SCF(Cdc4) are first subjected to prolyl isomerization, creating a signal for translocation and sequestration in the nucleolus, where they are then multiubiquitylated.
最近对一种SCF泛素连接酶在脯氨酰异构化而非泛素连接中所起作用的描述，引出了一种关于细胞周期蛋白E失活的两步模型，并且可能适用于哺乳动物细胞中其他SCF（Cdc4）靶标。在这个提出的模型中，SCF（Cdc4）的靶标首先经历脯氨酰异构化，产生一个信号使其易位并隔离在核仁中，然后在核仁中它们被多聚泛素化。
