用于评估单克隆抗-D抗体在预防恒河猴致敏中临床作用的淋巴细胞抗体依赖性细胞毒性试验。
Lymphocyte antibody-dependent cytotoxicity test for evaluation of clinical role of monoclonal anti-D-antibodies for prevention of rhesus sensitization.
作者信息
Olovnikova N I, Belkina E V, Nikolaeva T L, Miterev G Yu, Chertkov I L
机构信息
Hematology Research Center, Russian Academy of Medical Sciences, Moscow.
出版信息
Bull Exp Biol Med. 2006 Jan;141(1):57-61. doi: 10.1007/s10517-006-0093-4.
Monoclonal antibodies to D antigen were studied in the reaction of antibody-dependent cytotoxicity for evaluation of the possibility of using these antibodies for preventing rhesus sensitization. High hemolytic activity of four anti-D-monoclonal antibodies in the antibody-dependent cytotoxicity test, mediated by their interaction with FcgammaRI, and the capacity to accelerate elimination of D+ erythrocytes from circulation did not provide the immunosuppressive effect. It was hypothesized that monoclonal antibodies for prevention of rhesus sensitization should interact with FcgammaRIII on lymphocytes. These monoclonal antibodies are extremely rare: only 4 of 125 studied antibodies mediated hemolysis in the antibody-dependent cytotoxicity test with lymphocytes, while all polyclonal anti-D-preparations exhibited this activity.
研究了针对D抗原的单克隆抗体在抗体依赖性细胞毒性反应中的作用，以评估使用这些抗体预防恒河猴致敏的可能性。四种抗-D单克隆抗体在抗体依赖性细胞毒性试验中具有高溶血活性，通过它们与FcγRI的相互作用介导，并且具有加速从循环中清除D+红细胞的能力，但并未产生免疫抑制作用。据推测，用于预防恒河猴致敏的单克隆抗体应与淋巴细胞上的FcγRIII相互作用。这些单克隆抗体极为罕见：在与淋巴细胞进行的抗体依赖性细胞毒性试验中，125种研究的抗体中只有4种介导溶血，而所有多克隆抗-D制剂均表现出这种活性。
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