Dutta P, Bhattacharya J, Karak A K, Gupta Meenal, Chatterjee T, Srinivas U, Mishra P, Saxena R
Department of Haematology, All India Institute of Medical Sciences, New Delhi.
Indian J Pathol Microbiol. 2006 Apr;49(2):218-20.
Angiogenesis plays an important role in the pathogenesis of haematological neoplasms and may be correlated with the prognosis. We recently evaluated the microvessel densities in trephine biopsy sections of seventeen patients of myelodysplastic syndromes (MDS). Of the 17 cases, 2 were RAEB-t, 3 were RAEB, one was RARS and 11 were of the subtype RA (FAB subtyping). The microvessel counts were measured in the bone marrow biopsy sections by immunohistochemical staining, using CD34 reactive monoclonal antibodies. MVD was significantly higher in the cases of RAEB and RAEB-t as compared to the cases of RA. The average MVD per x400 in the cases of RA was 5.7 +/- 4.7 with a median value of 4.65 (range 19) whereas it was 45.4 +/- 10.0 and 44.0 (range 27.3) respectively in RAEB and RAEB-t (p <.001), the 95% confidence interval being (2.94, 8.5) and (36.6, 54.3), for the two groups respectively. This finding may imply that subtypes of MDS with a higher tendency for converting to acute leukaemia are associated with increased angiogenesis as compared to other subtypes where the risk of progression to acute leukaemia is much lower.
血管生成在血液系统肿瘤的发病机制中起重要作用，并且可能与预后相关。我们最近评估了17例骨髓增生异常综合征（MDS）患者骨髓活检切片中的微血管密度。17例患者中，2例为RAEB-t，3例为RAEB，1例为RARS，11例为RA亚型（FAB分型）。采用CD34反应性单克隆抗体，通过免疫组化染色在骨髓活检切片中测量微血管计数。与RA患者相比，RAEB和RAEB-t患者的微血管密度（MVD）显著更高。RA患者每400倍视野下的平均MVD为5.7±4.7，中位数为4.65（范围1 - 9），而RAEB和RAEB-t患者分别为45.4±10.0和44.0（范围27.3）（p <.001），两组的95%置信区间分别为(2.94, 8.5)和(36.6, 54.3)。这一发现可能意味着，与进展为急性白血病风险低的其他亚型相比，向急性白血病转化倾向较高的MDS亚型与血管生成增加有关。
