Abreu I, Costa I, Oliveira M, Cunha M, de Castro R
Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
Protoplasma. 2006 Aug;228(1-3):131-5. doi: 10.1007/s00709-006-0167-1. Epub 2006 Aug 31.
The cultivar Loureiro of Vitis vinifera is one of the most economically important, recommended in almost the totality of the Região Demarcada dos Vinhos Verdes. In vineyards, the grape productivity of this cultivar is normal while in others it is extremely low. The aim of this work was to study the morphology and germination of Vitis vinifera cv. Loureiro pollen with high and low productivity. The pollen grain was examined under light, transmission and scanning electron microscopy. Typically V. vinifera pollen present three furrows but in the cultivar Loureiro we found tricolporated and acolporated (without furrows or pores) pollen grains. Both pollen types present generative and vegetative cells with the usual aspect and a dense cytoplasm rich in organelles. In the acolporated pollen a continuous exine layer and an irregular intine layer were observed. Differences were found in the starch accumulation, since only in tricolporated pollen abundant plastids filled with numerous starch granules were observed. To determine the causes of the low productivity of this cultivar we tested pollen viability by the fluorochromatic reaction and pollen germinability by in vitro assays. We observed that the acolporated pollen grain is viable, but no germination was recorded.
酿酒葡萄品种洛雷罗是最重要的经济品种之一，在几乎整个绿酒法定产区都有推荐种植。在葡萄园里，该品种的葡萄产量正常，但在其他地方产量极低。这项工作的目的是研究高产量和低产量的酿酒葡萄品种洛雷罗花粉的形态和萌发情况。在光学显微镜、透射电子显微镜和扫描电子显微镜下对花粉粒进行了检查。典型的酿酒葡萄花粉有三条沟，但在洛雷罗品种中，我们发现了三孔沟和无孔沟（无沟或孔）的花粉粒。两种花粉类型都有生殖细胞和营养细胞，形态正常，细胞质浓密，富含细胞器。在无孔沟花粉中观察到连续的外壁层和不规则的内壁层。在淀粉积累方面发现了差异，因为仅在三孔沟花粉中观察到大量充满淀粉颗粒的质体。为了确定该品种低产的原因，我们通过荧光染色反应测试了花粉活力，并通过体外试验测试了花粉可萌发率。我们观察到无孔沟花粉粒是有活力的，但未记录到萌发情况。
