Li Min, Wang Si-Bao, Fan Mei-Zhen, Li Zeng-Zhi, Huang Yong-Ping
Anhui Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China.
Yi Chuan. 2006 Aug;28(8):977-83.
In the present paper, the genetic diversity of 48 Beauveria bassiana strains from different altitudes and at different seasons in Dabie Mountains of western Anhui was estimated using inter-simple sequence repeat (ISSR) markers. Twelve among 33 ISSR primers were chosen for their reproducibility and high polymorphism. Seven (2 - 11) markers per primer were scored, and a total of 84 fragments were amplified, in which 73 (81%) were polymorphic. Genetic diversity analysis revealed a relatively high level of intraspecific genetic diversity of B. bassiana in Dabie Mountains of western Anhui: the percentage of polymorphic loci (PPL) was 81%, Nei's genetic diversity (He) was 0.3187 and Shannon's genetic diversity index (I) was 0.4782. The genetic differentiation, Gst was 0.1028, indicating that a low degree of genetic differentiation occurred in the B. Bassiana among populations.
在本论文中,利用简单重复序列区间(ISSR)标记对来自皖西大别山不同海拔和不同季节的48株球孢白僵菌菌株的遗传多样性进行了评估。在33条ISSR引物中,选取了12条重复性好且多态性高的引物。每条引物记录7个(2 - 11)标记,共扩增出84条片段,其中73条(81%)具有多态性。遗传多样性分析表明,皖西大别山球孢白僵菌的种内遗传多样性水平相对较高:多态位点百分比(PPL)为81%,Nei氏遗传多样性(He)为0.3187,香农遗传多样性指数(I)为0.4782。遗传分化系数Gst为0.1028,表明球孢白僵菌种群间发生的遗传分化程度较低。