Miyazawa Masayuki, Kitadokoro Kengo, Kamitani Shigeki, Shime Hiroaki, Horiguchi Yasuhiko
Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita-shi, Osaka 565-0871, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt 9):906-8. doi: 10.1107/S1744309106030375. Epub 2006 Aug 18.
The C-terminal catalytic domain of Pasteurella multocida toxin, which is the virulence factor of the organism in P. multocida, has been expressed, purified and subsequently crystallized using the sitting-drop vapour-diffusion technique. Native diffraction data to 1.9 A resolution were obtained at the BL44XU beamline of SPring-8 from a flash-frozen crystal at 100 K. The crystals belong to space group C2, with unit-cell parameters a = 111.0, b = 150.4, c = 77.1 A, beta = 105.5 degrees, and are likely to contain one C-PMT (726 residues) per asymmetric unit.
多杀巴斯德氏菌毒素的C端催化结构域是该生物体的毒力因子,已通过坐滴气相扩散技术进行表达、纯化并随后结晶。在SPring-8的BL44XU光束线上,从100 K下快速冷冻的晶体获得了分辨率为1.9 Å的原生衍射数据。晶体属于空间群C2,晶胞参数为a = 111.0、b = 150.4、c = 77.1 Å、β = 105.5°,每个不对称单元可能包含一个C-PMT(726个残基)。