Venazzi Eneide Aparecida Sabaini, Roberto Andréa Claudia Bekner Silva, Barbosa-Tessmann Ione Parra, Zanzarini Paulo Donizeti, Lonardoni Maria Valdrinez Campana, Silveira Thaís Gomes Verzignassi
Departamento de Análises Clínicas, Departamento de Bioquímica, Universidade Estadual de Maringá, Maringá, PR, Brasil.
Mem Inst Oswaldo Cruz. 2006 Jun;101(4):427-30. doi: 10.1590/s0074-02762006000400014.
The objective of this work was to compare the polymerase chain reaction (PCR) using lesion scrapping with other conventional techniques for the diagnosis of the American tegumentary leishmaniasis (ATL). For this, patients with cutaneous lesions suspected to be ATL were studied. The DNA was amplified with the MP1L/MP3H primers. From the 156 studied patients, 79 (50.6%) presented positive parasite direct search (PD), 81 (51.9%) had positive Montenegro skin test (MST), and 90 (57.7%) presented PD and/or MST positive. The PCR was positive in all of the positive-PD patients (100% sensitivity), in 91.1% of the positive PD and/or MST patients, and in 27.3% of the patients that presented negative PD and positive MST. The PCR positivity was similar to the PD (P = 0.2482) and inferior to the MST (P = 0.0455), and to the PD/MST association (P = 0.0133). The high PCR sensitivity, and positivity in those cases where the PD was negative, highlights the importance of this technique as an auxiliary tool for the diagnosis of ATL.
这项工作的目的是比较使用病变刮片的聚合酶链反应（PCR）与其他传统技术在诊断美洲皮肤利什曼病（ATL）方面的效果。为此，对疑似患有ATL皮肤病变的患者进行了研究。使用MP1L/MP3H引物扩增DNA。在156名研究患者中，79名（50.6%）寄生虫直接检测（PD）呈阳性，81名（51.9%） Montenegro皮肤试验（MST）呈阳性，90名（57.7%）PD和/或MST呈阳性。所有PD阳性患者的PCR均为阳性（敏感性100%），在PD和/或MST阳性患者中91.1%呈阳性，在PD阴性但MST阳性的患者中27.3%呈阳性。PCR阳性率与PD相似（P = 0.2482），低于MST（P = 0.0455），也低于PD/MST联合检测（P = 0.0133）。PCR的高敏感性以及在PD为阴性的病例中的阳性结果，突出了该技术作为ATL诊断辅助工具的重要性。
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