Viranaicken Wildriss, Gasmi Laïla, Chauvin Céline, Denoulet Philippe, Larcher Jean-Christophe
Laboratoire de Biochimie Cellulaire, UMR 7098 CNRS, Université Pierre et Marie Curie, 9 Quai Saint-Bernard, Case 265, 75252 Paris Cedex 05, France.
Genomics. 2006 Nov;88(5):622-32. doi: 10.1016/j.ygeno.2006.08.006. Epub 2006 Sep 6.
The mammalian IlF3 and NF90 proteins, involved in several cellular functions, have common N-terminal and central sequences and specific C-terminal regions. These proteins exhibit a large heterogeneity generated by posttranscriptional and posttranslational modifications. Part of their polymorphism is due to the alternative splicing of exon 3 located just downstream of the translation initiation codon. This 39-nucleotide-long exon, not described so far, codes for an N-terminal sequence of 13 residues (ALYHHHFITRRRR) also present in rat and human IlF3 or NF90. Four mRNAs are expressed in mouse brain, two for Ilf3 and two for NF90, differing in their 3' sequence to generate the specific Ilf3 and NF90 C-terminal domains and in the presence or the absence of exon 3 to generate long and short isoforms of both proteins. By RT-PCR, no other variants were found. Combining our results and GenBank sequences, we determined the exon-intron organization of the entire mouse Ilf3 gene divided into 22 exons.
参与多种细胞功能的哺乳动物IlF3和NF90蛋白具有共同的N端和中央序列以及特定的C端区域。这些蛋白表现出由转录后和翻译后修饰产生的高度异质性。它们部分多态性归因于位于翻译起始密码子下游的外显子3的可变剪接。这个长度为39个核苷酸的外显子，迄今尚未被描述，编码一个由13个残基组成的N端序列（ALYHHHFITRRRR），该序列也存在于大鼠和人类的IlF3或NF90中。在小鼠大脑中表达四种mRNA，两种用于Ilf3，两种用于NF90，它们的3'序列不同以产生特定的Ilf3和NF90 C端结构域，并且外显子3的存在与否产生这两种蛋白的长和短异构体。通过逆转录聚合酶链反应（RT-PCR），未发现其他变体。结合我们的结果和GenBank序列，我们确定了整个小鼠Ilf3基因的外显子-内含子组织，该基因分为22个外显子。
