鱼腥藻PCC 7120发育异形胞中ntcA调控基因的局部诱导。
Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120.
作者信息
Olmedo-Verd Elvira, Muro-Pastor Alicia M, Flores Enrique, Herrero Antonia
机构信息
Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Américo Vespucio 49, E-41092 Seville, Spain.
出版信息
J Bacteriol. 2006 Sep;188(18):6694-9. doi: 10.1128/JB.00509-06.
Abstract
The ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N(2)-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a complex promoter region that includes a constitutive promoter (P(2)) and promoters that are induced upon N step-down (P(1) and P(3)). Expression of ntcA was investigated with the use of an ntcA-gfp translational fusion, which was introduced both in the natural ntcA locus and in a heterologous genomic place. Induction of ntcA-gfp took place after N step-down in all the cells of the filament, but at especially high levels in developing heterocysts. Localized induction could be driven independently by P(3) and P(1).
摘要
ntcA基因在蓝细菌中编码一种N调控转录调节因子。在固氮、形成异形胞的蓝细菌鱼腥藻属Anabaena sp.菌株PCC 7120中，ntcA是一个自调控基因，它从一个复杂的启动子区域转录，该区域包括一个组成型启动子（P(2)）和在氮素水平下降时被诱导的启动子（P(1)和P(3)）。使用ntcA - gfp翻译融合体对ntcA的表达进行了研究，该融合体被引入到天然ntcA基因座和异源基因组位置。ntcA - gfp在氮素水平下降后在丝状体的所有细胞中被诱导，但在发育中的异形胞中诱导水平特别高。局部诱导可由P(3)和P(1)独立驱动。
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