一种与乳腺癌相关基因BARD1同源的基因参与植物的DNA修复。
A homologue of the breast cancer-associated gene BARD1 is involved in DNA repair in plants.
作者信息
Reidt Wim, Wurz Rebecca, Wanieck Kristina, Chu Hoang Ha, Puchta Holger
机构信息
Botanisches Institut II, Universität Karlsruhe, Karlsruhe, Germany.
出版信息
EMBO J. 2006 Sep 20;25(18):4326-37. doi: 10.1038/sj.emboj.7601313. Epub 2006 Sep 7.
Abstract
hBRCA1 and hBARD1 are tumor suppressor proteins that are involved as heterodimer via ubiquitinylation in many cellular processes, such as DNA repair. Loss of BRCA1 or BARD1 results in early embryonic lethality and chromosomal instability. The Arabidopsis genome carries a BRCA1 homologue, and we were able to identify a BARD1 homologue. AtBRCA1 and the putative AtBARD1 protein are able to interact with each other as indicated by in vitro and in planta experiments. We have identified T-DNA insertion mutants for both genes, which show no visible phenotype under standard growth conditions and are fully fertile. Thus, in contrast to animals, both genes have no indispensable role during development and meiosis in plants. The two single as well as the double mutant are to a similar extent sensitive to mitomycin C, indicating an epistatic interaction in DNA crosslink repair. We could further demonstrate that in Arabidopsis BARD1 plays a prominent role in the regulation of homologous DNA repair in somatic cells.
摘要
人源BRCA1和人源BARD1是肿瘤抑制蛋白，它们以异源二聚体的形式通过泛素化参与许多细胞过程，如DNA修复。BRCA1或BARD1的缺失会导致早期胚胎致死和染色体不稳定。拟南芥基因组携带一个BRCA1同源物，我们也能够鉴定出一个BARD1同源物。体外和植物体内实验表明，拟南芥BRCA1（AtBRCA1）和假定的AtBARD1蛋白能够相互作用。我们已经鉴定出这两个基因的T-DNA插入突变体，它们在标准生长条件下没有可见的表型，并且完全可育。因此，与动物不同，这两个基因在植物发育和减数分裂过程中没有不可或缺的作用。单突变体和双突变体对丝裂霉素C的敏感程度相似，这表明在DNA交联修复中存在上位性相互作用。我们还可以进一步证明，在拟南芥中，BARD1在体细胞同源DNA修复的调控中起着重要作用。
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