Miller J S, Quarles J M
Department of Medical Microbiology and Immunology, College of Medicine, Texas A&M University, College Station 77843-1114.
Cytometry. 1990;11(6):667-75. doi: 10.1002/cyto.990110603.
The identification of microorganisms by flow cytometry was evaluated by using a double staining technique with propidium iodide and fluorescein isothiocyanate and a two dimensional analysis. A diverse group of 19 different species and strains of microorganisms was tested to determine if they could be differentiated by flow cytometry. The organisms tested displayed characteristic and distinct two dimensional fluorescent patterns which allowed ready grouping and differentiation into subsets of organisms. The slopes and correlation coefficients of the histograms and the ratio of red to green signals expressed these differences quantitatively and allowed organisms to be placed into one of three groups based on these values. In some instances, as with Streptococcus pneumoniae and pyogenes and Staphylococcus aureus and epidermidis, it was possible to distinguish between species of bacteria from the same genus. The use of dual dye labeling and flow cytometry provided a rapid method of identifying selected microorganisms and may be broadly applicable for the detection and identification of many bacteria and fungi.
采用碘化丙啶和异硫氰酸荧光素双重染色技术及二维分析方法，对通过流式细胞术鉴定微生物进行了评估。测试了19种不同种类和菌株的多种微生物，以确定它们是否可通过流式细胞术进行区分。所测试的微生物呈现出特征性且独特的二维荧光模式，这使得能够轻松地将它们分组并区分为不同的微生物子集。直方图的斜率和相关系数以及红/绿信号比值对这些差异进行了定量表达，并能根据这些值将微生物分为三组之一。在某些情况下，如肺炎链球菌与化脓性链球菌以及金黄色葡萄球菌与表皮葡萄球菌，有可能区分同一属内的细菌种类。使用双染料标记和流式细胞术提供了一种快速鉴定特定微生物的方法，并且可能广泛适用于许多细菌和真菌的检测与鉴定。
