Pal M, Hasegawa A, Ono K, Lee C W
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Nihon Juigaku Zasshi. 1990 Jun;52(3):527-31. doi: 10.1292/jvms1939.52.527.
A new staining solution named "PHOL" is described for studying the morphology of clinical and environmental isolates of fungi and Prototheca. In total 69 isolates were used for assessing the efficacy of this newly formulated stain. The solution has shown good ability to stain all 62 isolates of fungi and 7 of Prototheca, and thus helped in the identification of the organisms. The new solution has the potential to stain the young as well as old isolates of fungi and Prototheca. It is emphasized that a wider application of this newly introduced inexpensive and safe staining solution will help the mycologists to study the morphological characteristics of yeast, molds, and Prototheca obtained from man, animals and environment.
一种名为“PHOL”的新型染色液被用于研究临床和环境中分离出的真菌及原藻的形态。总共69株分离菌株被用于评估这种新配制染色液的效果。该染色液对所有62株真菌和7株原藻均显示出良好的染色能力,从而有助于对这些微生物进行鉴定。这种新染色液有潜力对真菌和原藻的新老分离菌株进行染色。需要强调的是,这种新推出的廉价且安全的染色液的广泛应用将有助于真菌学家研究从人、动物和环境中获得的酵母、霉菌及原藻的形态特征。