谷子（Setaria italica (L.) P. Beauv.）核糖体DNA基因间隔序列及其特征与在谷子地方品种分型中的应用

Ribosomal DNA intergenic spacer sequence in foxtail millet, Setaria italica (L.) P. Beauv. and its characterization and application to typing of foxtail millet landraces.

作者信息

Fukunaga Kenji, Ichitani Katsuyuki, Taura Satoru, Sato Muneharu, Kawase Makoto

机构信息

Laboratory of Crop Evolution, Faculty of Agriculture, Kyoto University, Mukoh, Japan.

出版信息

Hereditas. 2005 Feb;142(2005):38-44. doi: 10.1111/j.1601-5223.2005.01905.x.

DOI:10.1111/j.1601-5223.2005.01905.x

PMID:16970610

Abstract

We determined the sequence of ribosomal DNA (rDNA) intergenic spacer (IGS) of foxtail millet isolated in our previous study, and identified subrepeats in the polymorphic region. We also developed a PCR-based method for identifying rDNA types based on sequence information and assessed 153 accessions of foxtail millet. Results were congruent with our previous works. This study provides new findings regarding the geographical distribution of rDNA variants. This new method facilitates analyses of numerous foxtail millet accessions. It is helpful for typing of foxtail millet germplasms and elucidating the evolution of this millet.

摘要

我们测定了在之前研究中分离得到的谷子核糖体DNA（rDNA）基因间隔区（IGS）的序列，并在多态性区域鉴定出亚重复序列。我们还基于序列信息开发了一种用于鉴定rDNA类型的PCR方法，并对153份谷子种质进行了评估。结果与我们之前的研究一致。本研究提供了有关rDNA变异地理分布的新发现。这种新方法有助于对大量谷子种质进行分析。它有助于谷子种质的分型和阐明这种谷子的进化。