Souri Effat, Jalalizadeh Hassan, Kebriaee-Zadeh Abbas, Shekarchi Maral, Dalvandi Afshin
Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), Iran.
Biomed Chromatogr. 2006 Dec;20(12):1309-14. doi: 10.1002/bmc.697.
A new, simple, and reproducible method for determination of carboxylic acid metabolite of clopidogrel in human plasma has been developed. After liquid-liquid extraction in acidic medium with chloroform, samples were quantified on a Nova-pak C(8), 5 microm column using a mixture of 30 mM K(2)HPO(4)-THF-acetonitrile (pH = 3, 79:2:19, v/v/v) as mobile phase with UV detection at 220 nm. The flow rate was set at 0.9 mL/min. Ticlopidine was used as internal standard and the total run time of analysis was about 12 min. The method was linear over the range of 0.2-10 microg/mL of clopidogrel metabolite in plasma (r(2) > 0.999). The within-day and between-day precision values were in the range 1.0-4.8%. The limit of quantification of the method was 0.2 microg/mL. The method was successfully used to study the pharmacokinetics of clopidogrel in healthy volunteers.
已开发出一种用于测定人血浆中氯吡格雷羧酸代谢物的新的、简单且可重复的方法。在酸性介质中用氯仿进行液液萃取后，样品在Nova-pak C(8)、5微米柱上进行定量分析，使用30 mM K(2)HPO(4)-四氢呋喃-乙腈（pH = 3，79:2:19，v/v/v）的混合物作为流动相，在220 nm处进行紫外检测。流速设定为0.9 mL/min。噻氯匹定用作内标，分析的总运行时间约为12分钟。该方法在血浆中氯吡格雷代谢物浓度为0.2 - 10微克/毫升范围内呈线性（r(2) > 0.999）。日内和日间精密度值在1.0 - 4.8%范围内。该方法的定量限为0.2微克/毫升。该方法已成功用于研究健康志愿者体内氯吡格雷的药代动力学。
