Stabilization of glucocorticoid-receptor interactions in vitro by removal of RNA bound to receptor complexes in vivo.

The dissociation of the steroid from glucocorticoid-receptor-RNA complexes at 5 degrees C was evaluated in cytosolic and nuclear extracts prepared from Hela cells crosslinked in vivo with glutaraldehyde. Sample treatment with catalytically active RNase A prevented the dissociation of the steroid which was induced by sample dilution with buffer. Dilution of the extracts with boiled cytosol, instead, stabilized steroid-receptor interactions. We conclude that some heat-stable factor should be also associated with glucocorticoid-receptor-RNA complexes from crosslinked cells, stabilizing steroid-receptor interactions, and we propose that it could counteract the labilizing effect of RNA.