通过离线固相萃取-液相色谱-电喷雾串联质谱法测定三刺鱼（Gasterosteus aculeatus）大脑中的神经肽精氨酸加压催产素和异催产素。
Determination of the neuropeptides arginine vasotocin and isotocin in brains of three-spined sticklebacks (Gasterosteus aculeatus) by off-line solid phase extraction-liquid chromatography-electrospray tandem mass spectrometry.
作者信息
Kleszczyńska Agnieszka, Dumont Emmie, Cornelis Rita, Gozdowska Magdalena, Kulczykowska Ewa, Sandra Pat
机构信息
Department of Genetics and Marine Biotechnology, Institute of Oceanology of Polish Academy of Sciences, Powstańców Warszawy 55 Str, 81-712 Sopot, Poland.
出版信息
J Chromatogr A. 2007 May 25;1150(1-2):290-4. doi: 10.1016/j.chroma.2006.08.084. Epub 2006 Sep 25.
A method based on solid phase extraction (SPE) followed by liquid chromatography-electrospray ionisation tandem mass spectrometry for the determination of the nonapeptides arginine vasotocin (AVT) and isotocin (IT) in brains of three-spined sticklebacks (Gasterosteus aculeatus) is described. Separation and detection were optimized using synthetic standards. Limits of detection (LOD) for standard solutions were 160 pg mL(-1) for AVT and 250 pg mL(-1) for IT. The SPE procedure hardly affected the LODs for standard solutions. Mainly because of ion suppression, LODs for AVT and IT in brains were approximately 5 and 25 pg mg(-1), respectively. The concentrations determined in the brain of several fishes ranged from 10 to 500 pg mg(-1) for AVT and from 400 to 4000 pg mg(-1) for IT.
本文描述了一种基于固相萃取（SPE），随后进行液相色谱 - 电喷雾电离串联质谱法来测定三刺鱼（Gasterosteus aculeatus）脑中九肽精氨酸加压催产素（AVT）和异催产素（IT）的方法。使用合成标准品对分离和检测进行了优化。标准溶液中AVT的检测限（LOD）为160 pg mL⁻¹，IT为250 pg mL⁻¹。SPE程序对标准溶液的检测限影响不大。主要由于离子抑制作用，脑中AVT和IT的检测限分别约为5 pg mg⁻¹和25 pg mg⁻¹。在几种鱼的脑中测定的AVT浓度范围为10至500 pg mg⁻¹，IT为400至4000 pg mg⁻¹。
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