Bose Bipasha, Gour Rekha R, Motiwale Leena, Rao K V K
Chemical Carcinogenesis Group, Khanolkar Shodhika, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, India.
Indian J Exp Biol. 2006 Sep;44(9):693-8.
Malachite green (MG) induces DNA damage and malignant transformation of Syrian hamster embryo (SHE) cells in primary culture. In the present study, we have studied the role of all the three isoforms of mitogen activated protein (MAP) kinases i.e. ERK (extracellular regulated kinase), JNK (JUN- N- terminal kinase) and p38 kinase during transformation of SHE cells by MG. The results showed that transformed cells were associated with a decreased expression of phosphoactive ERK and JNK and increased expression of p38 kinase as evident from the Western blot, immunofluorescence and flow cytometry studies. Also, a persistent nuclear localization of p38 kinase was observed in the transformed cells. The present study indicated that p38 kinase was present at higher levels and seemed to be associated with transformation, which suggested that inhibitors of p38 kinase could serve in general as potential agents for selective cancer therapy.
孔雀石绿（MG）可诱导原代培养的叙利亚仓鼠胚胎（SHE）细胞发生DNA损伤和恶性转化。在本研究中，我们研究了丝裂原活化蛋白（MAP）激酶的三种同工型，即细胞外调节激酶（ERK）、JUN-N端激酶（JNK）和p38激酶在MG诱导SHE细胞转化过程中的作用。结果表明，从蛋白质免疫印迹、免疫荧光和流式细胞术研究中可以看出，转化细胞中磷酸化活性ERK和JNK的表达降低，而p38激酶的表达增加。此外，在转化细胞中观察到p38激酶持续定位于细胞核。本研究表明，p38激酶水平较高且似乎与细胞转化有关，这表明p38激酶抑制剂通常可作为选择性癌症治疗的潜在药物。
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