与哺乳动物细胞中的睡美人转座子、Tol2转座子和Mos1转座子相比，piggyBac是一种灵活且高度活跃的转座子。
piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells.
作者信息
Wu Sareina Chiung-Yuan, Meir Yaa-Jyuhn James, Coates Craig J, Handler Alfred M, Pelczar Pawel, Moisyadi Stefan, Kaminski Joseph M
机构信息
Cancer Research Center, Medical College of Georgia, Augusta, GA 30912, USA.
出版信息
Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15008-13. doi: 10.1073/pnas.0606979103. Epub 2006 Sep 27.
Abstract
A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic integration efficiencies of piggyBac, hyperactive Sleeping Beauty (SB11), Tol2, and Mos1 in four mammalian cell lines. piggyBac demonstrated significantly higher transposition activity in all cell lines whereas Mos1 had no activity. Furthermore, piggyBac transposase coupled to the GAL4 DNA-binding domain retains transposition activity whereas similarly manipulated gene products of Tol2 and SB11 were inactive. The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis.
摘要
对于转基因的许多应用（包括基因治疗）而言，一种用于高效位点特异性整合的非病毒载体将是很理想的。在本研究中，我们直接比较了猪尾巴（piggyBac）、超活性睡美人（SB11）、Tol2和Mos1在四种哺乳动物细胞系中的基因组整合效率。猪尾巴在所有细胞系中均表现出显著更高的转座活性，而Mos1没有活性。此外，与GAL4 DNA结合结构域偶联的猪尾巴转座酶保留了转座活性，而Tol2和SB11经类似操作的基因产物则无活性。猪尾巴的高转座活性及其转座酶分子修饰的灵活性表明，有可能将其常规用于哺乳动物转基因。
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