在替代铜绿假单胞菌菌株中调控单拷贝外排泵基因表达的方法：鉴定伯克霍尔德菌1026b的BpeEF-OprC氯霉素和甲氧苄啶外排泵
Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b.
作者信息
Kumar Ayush, Chua Kim-Lee, Schweizer Herbert P
机构信息
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA.
出版信息
Antimicrob Agents Chemother. 2006 Oct;50(10):3460-3. doi: 10.1128/AAC.00440-06.
Abstract
Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump.
摘要
重组mini-Tn7表达载体构建并整合到替代的、外排敏感且生物安全的铜绿假单胞菌宿主染色体中，这一方法被验证为研究未表征细菌外排泵的通用方法。利用该方法，已证明伯克霍尔德菌bpeEF-oprC操纵子编码氯霉素和甲氧苄啶外排泵。
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