双量子滤波旋转回波双共振

Double-quantum filtered rotational-echo double resonance.

作者信息

Matsuoka Shigeru, Schaefer Jacob

机构信息

Department of Chemistry, Washington University, St. Louis, MO 63130, USA.

出版信息

J Magn Reson. 2006 Dec;183(2):252-8. doi: 10.1016/j.jmr.2006.08.010. Epub 2006 Sep 27.

DOI:10.1016/j.jmr.2006.08.010

PMID:17008112

Abstract

The homonuclear scalar coupling of a directly bonded 13C-13C pair has been used to create a double-quantum filter (DQF) to remove the natural-abundance 13C background in 13C{15N} rotational-echo double-resonance (REDOR) experiments. The DQF scalar and REDOR dipolar evolution periods are coincident which is important for sensitivity in the event of weak 13C-15N dipolar coupling. Calculated and observed 13C{15N} DQF-REDOR dephasings were in agreement for a test sample of mixed recrystallized labeled alanines. Glycine metabolism in a single uniform-15N soybean leaf labeled for 6 min by 13CO2 was measured quantitatively by 13C{15N} DQF-REDOR with no background interferences.

摘要

直接相连的13C-13C对的同核标量耦合已被用于创建一个双量子滤波器（DQF），以在13C{15N}旋转回波双共振（REDOR）实验中去除天然丰度的13C背景。DQF标量和REDOR偶极演化期是重合的，这对于弱13C-15N偶极耦合情况下的灵敏度很重要。对于混合重结晶标记丙氨酸的测试样品，计算得到的和观察到的13C{15N} DQF-REDOR去相位是一致的。通过13C{15N} DQF-REDOR对用13CO2标记6分钟的单一均匀15N大豆叶片中的甘氨酸代谢进行了定量测量，且无背景干扰。