邻苯二甲酸二（2-乙基己基）酯对新生KM小鼠睾丸及睾丸间质细胞的影响

[Di(2-ethylhexyl) phthalate affects the testes and leydig cells of neonatal KM mice].

作者信息

Song Xiao-feng, Wei Guang-hui, Deng Yong-ji, Chen Xuan, Liu Xing, Zhang De-ying

机构信息

Department of Urology, Children's Hospital, Chongqing University of Medical Sciences, Chongqing 400014, China.

出版信息

Zhonghua Nan Ke Xue. 2006 Sep;12(9):775-9.

PMID:17009524

Abstract

OBJECTIVE

To explore the effects of di(2-ethylhexyl)phthalate (DEHP) on neonatal mice's testes and Leydig cells in vivo.

METHODS

Pregnant mice were exposed to DEHP at the dose of 100 mg/kg, 200 mg/kg or 500 mg/kg (body weight) per day by gavage from gestation day 12 (GD 12) through postnatal day 3 (PND 3), respectively. The testis and body weights, testicular histopathology and the activity of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) of the neonatal mice were investigated.

RESULTS

The body and testis weights of the male mice's offspring were significantly reduced following DEHP exposure. Leydig cell morphology was affected significantly by DEHP as compared with the controls. Leydig cells obviously increased in the neonatal mice's testes on PND 15 and PND 30 when exposed to DEHP (500 mg/[kg x d]). Activities and positive area of the steroidogenic enzymes 3beta-HSD immunoexpression decreased markedly when exposed to DEHP (100 mg/[kg x d] or 200 mg/[kg x d]). Image analysis showed a decrease in the activities of 3beta-HSD in the animals exposed to DEHP (500 mg/[kg x d]), but an increase in the positive area of 3beta-HSD immunoexpression as compared with the control animals on PND 15 (P < 0.01).

CONCLUSION

DEHP affects the Leydig cell morphology, the activity of 3beta-HSD, the testis and body weights and the testicular histopathology of neonatal mice, and it may function as an antiandrogenic agent.

摘要

目的

探讨邻苯二甲酸二（2-乙基己基）酯（DEHP）对新生小鼠睾丸及睾丸间质细胞的体内影响。

方法

从妊娠第12天（GD 12）至出生后第3天（PND 3），分别以100 mg/kg、200 mg/kg或500 mg/kg（体重）的剂量通过灌胃使妊娠小鼠暴露于DEHP。研究新生小鼠的睾丸和体重、睾丸组织病理学以及3β-羟基类固醇脱氢酶（3β-HSD）的活性。

结果

DEHP暴露后，雄性小鼠后代的体重和睾丸重量显著降低。与对照组相比，DEHP对睾丸间质细胞形态有显著影响。暴露于DEHP（500 mg/[kg·d]）时，新生小鼠在出生后第15天和第30天睾丸中的睾丸间质细胞明显增多。暴露于DEHP（100 mg/[kg·d]或200 mg/[kg·d]）时，类固醇生成酶3β-HSD免疫表达的活性和阳性面积显著降低。图像分析显示，暴露于DEHP（500 mg/[kg·d]）的动物中3β-HSD的活性降低，但在出生后第15天与对照动物相比，3β-HSD免疫表达的阳性面积增加（P < 0.01）。