顺磁珠在纯化炭疽芽孢杆菌保护性抗原中的应用。
Application of paramagnetic beads for purifying Bacillus anthracis protective antigen.
作者信息
Zarzecka A, Bartoszcze M
机构信息
Military Institute of Hygiene and Epidemiology, Biological Threats Identification and Countering Center, 24-100 Pulawy, Lubelska 2, Poland.
出版信息
J Vet Med B Infect Dis Vet Public Health. 2006 Oct;53(8):393-4. doi: 10.1111/j.1439-0450.2006.00970.x.
Paramagnetic beads coated with Protein G and Tosylactivated-280 dynabeads have been used to purify Bacillus anthracis protective antigen from a liquid culture. The obtained protein was used in the enzyme-linked immunosorbent assay test to detect B. anthracis protective antigen antibodies in human sera collected from immunized individuals. The purification method using paramagnetic beads is very effective. It is fast, easy and may be carried out practically in any laboratory.
包被有蛋白G的顺磁珠和对甲苯磺酰活化的280磁珠已被用于从液体培养物中纯化炭疽芽孢杆菌保护性抗原。所获得的蛋白质用于酶联免疫吸附测定试验，以检测从免疫个体采集的人血清中的炭疽芽孢杆菌保护性抗原抗体。使用顺磁珠的纯化方法非常有效。它快速、简便，几乎可以在任何实验室进行。
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