猪心羰基还原酶的结晶及初步X射线晶体学研究
Crystallization and preliminary X-ray crystallographic studies of pig heart carbonyl reductase.
作者信息
Aoki Ken-ichi, Tanaka Nobutada, Ishikura Shuhei, Araki Naoko, Imamura Yorishige, Hara Akira, Nakamura Kazuo T
机构信息
School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt 10):1037-40. doi: 10.1107/S1744309106037535. Epub 2006 Sep 30.
Pig heart carbonyl reductase (PHCR), which belongs to the short-chain dehydrogenase/reductase (SDR) family, has been crystallized by the hanging-drop vapour-diffusion method. Two crystal forms (I and II) have been obtained in the presence of NADPH. Form I crystals belong to the tetragonal space group P4(2), with unit-cell parameters a = b = 109.61, c = 94.31 A, and diffract to 1.5 A resolution. Form II crystals belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 120.10, c = 147.00 A, and diffract to 2.2 A resolution. Both crystal forms are suitable for X-ray structure analysis at high resolution.
猪心脏羰基还原酶（PHCR）属于短链脱氢酶/还原酶（SDR）家族，已通过悬滴气相扩散法结晶。在存在NADPH的情况下获得了两种晶型（I和II）。I型晶体属于四方晶系空间群P4(2)，晶胞参数a = b = 109.61，c = 94.31 Å，衍射分辨率为1.5 Å。II型晶体属于四方晶系空间群P4(1)2(1)2，晶胞参数a = b = 120.10，c = 147.00 Å，衍射分辨率为2.2 Å。两种晶型都适用于高分辨率X射线结构分析。
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