Navarro-Hernández Rosa Elena, Oregón-Romero Edith, Rangel-Villalobos Héctor, Vázquez-Del Mercado Mónica, Ruiz-Quezada Sandra Luz, Maldonado-González Monserrat, Torres-Vitela Refugio, Muñoz-Valle José Francisco
Doctorado en Ciencias Biomédicas, Orientación en Inmunología-Laboratorio de Inmunología, Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, Centro Universitario de Ciencias de la Salud, Jalisco, Mexico.
J Rheumatol. 2006 Oct;33(10):1968-72.
To investigate the relationship of A561C polymorphism and sE-selectin levels with rheumatoid arthritis (RA) clinical activity.
In a case-control study, we compared 60 patients with RA and 60 healthy subjects. Patients fulfilled the 1987 American College of Rheumatology criteria. Soluble E-selectin levels were measured from serum samples using the ELISA kit. We investigated E-selectin A561C polymorphism by the restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique. The disease activity was recorded with Spanish Health Assessment Questionnaire Disability Index (HAQ-DI), Spanish Arthritis Impact Measurement Scales (AIMS), and Disease Activity Score (DAS28) scores. A p value < 0.05 was considered significant.
Patients with RA showed higher sE-selectin levels than controls (mean 91.7 vs 39 ng/ml; p = 0.002). A positive correlation between sE-selectin and rheumatoid factor (RF), erythrocyte sedimentation rate (ESR), Spanish HAQ-DI, and DAS28 scores was found. The E-selectin polymorphism analysis showed diminished frequency in RA of heterozygous A/C genotype and increased frequency of homozygous wild-type A/A genotype (p = 0.043, OR 1.45; 95% CI 1.125-16.167) versus A/C and A/A genotype in healthy subjects. No significant association between A561C polymorphism and clinical activity was present.
The sE-selectin, RF, and ESR, in addition to clinical indices, were associated with clinical activity in RA. We highlighted the presence of A/A genotype A561C polymorphism in our patients with RA.
探讨A561C多态性及可溶性E选择素（sE-selectin）水平与类风湿关节炎（RA）临床活动度的关系。
在一项病例对照研究中，我们比较了60例RA患者和60名健康受试者。患者符合1987年美国风湿病学会标准。使用酶联免疫吸附测定（ELISA）试剂盒从血清样本中检测可溶性E选择素水平。我们采用限制性片段长度多态性-聚合酶链反应（RFLP-PCR）技术研究E选择素A561C多态性。用西班牙健康评估问卷残疾指数（HAQ-DI）、西班牙关节炎影响测量量表（AIMS）和疾病活动评分（DAS28）记录疾病活动度。p值<0.05被认为具有统计学意义。
RA患者的sE选择素水平高于对照组（平均91.7对39 ng/ml；p = 0.002）。发现sE选择素与类风湿因子（RF）、红细胞沉降率（ESR）、西班牙HAQ-DI和DAS28评分之间呈正相关。E选择素多态性分析显示，与健康受试者的A/C和A/A基因型相比，RA患者中杂合子A/C基因型的频率降低，纯合野生型A/A基因型的频率增加（p = 0.043，比值比1.45；95%可信区间1.125 - 16.167）。A561C多态性与临床活动度之间无显著关联。
除临床指标外，sE选择素、RF和ESR与RA的临床活动度相关。我们强调了RA患者中存在A561C多态性的A/A基因型。
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