N，N-二甲基甲酰胺对光合磷酸化作用和电子传递的抑制作用

Inhibition of photophosphorylation and electron transport by N,N-dimethylformamide.

作者信息

Pe Na-Valdivia C B, Rodriguez-Flores L, de Gómez-Puyou M T, Lotina-Hennsen B

机构信息

Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, 04510 México, D.F. Mexico.

出版信息

Biophys Chem. 1991 Nov;41(2):169-74. doi: 10.1016/0301-4622(91)80016-k.

DOI:10.1016/0301-4622(91)80016-k

PMID:17014794

Abstract

The basal electron transport of pea chloroplasts was inhibited by 78% by 7% (v/v) N,N-dimethylformamide; the inhibition was partially reversed by NH4Cl. N,N-Dimethylformamide also inhibited the Pi-ATP exchange, ATP synthesis and to a smaller extent Mg2+-ATPase activity. Light induced proton uptake was not affected by up to 30% (v/v) N,N-dimethylformamide. Uncoupled electron transport in photosystem II was inhibited to a larger extent by N,N-dimethylformamide than in photosystem I. These results indicate that N,N-dimethylformamide acts as an inhibitor of energy transfer and electron transport.

摘要

7%（v/v）的N，N-二甲基甲酰胺可使豌豆叶绿体的基础电子传递受到78%的抑制；氯化铵可部分逆转这种抑制作用。N，N-二甲基甲酰胺还抑制磷酸-ATP交换、ATP合成，并在较小程度上抑制Mg2+-ATP酶活性。高达30%（v/v）的N，N-二甲基甲酰胺对光诱导的质子摄取没有影响。与光系统I相比，光系统II中解偶联的电子传递受到N，N-二甲基甲酰胺的抑制程度更大。这些结果表明，N，N-二甲基甲酰胺是能量传递和电子传递的抑制剂。