Król Jarosław E, Mazur Andrzej, Marczak Małgorzata, Skorupska Anna
Department of General Microbiology, Institute of Microbiology and Biotechnology, University of Maria Curie Skłodowska, 19 Akademicka Street, 20-033 Lublin, Poland.
Genomics. 2007 Feb;89(2):237-47. doi: 10.1016/j.ygeno.2006.08.015. Epub 2006 Oct 2.
We applied a genomic approach in the identification of genes required for the biosynthesis of different polysaccharides in Rhizobium leguminosarum bv. trifolii TA1 (RtTA1). Pulsed-field gel electrophoresis analyses of undigested genomic DNA revealed that the RtTA1 genome is partitioned into a chromosome and four large plasmids. The combination of sequencing of RtTA1 library BAC clones and PCR amplification of polysaccharide genes from the RtTA1 genome led to the identification of five large regions and clusters, as well as many separate potential polysaccharide biosynthesis genes dispersed in the genome. We observed an apparent abundance of genes possibly linked to lipopolysaccharide biosynthesis. All RtTA1 polysaccharide biosynthesis regions showed a high degree of conserved synteny between R. leguminosarum bv. viciae and/or Rhizobium etli. A majority of the genes displaying a conserved order also showed high sequence identity levels.
我们采用基因组学方法来鉴定三叶草根瘤菌TA1（RtTA1）中不同多糖生物合成所需的基因。对未消化的基因组DNA进行脉冲场凝胶电泳分析表明，RtTA1基因组被划分为一条染色体和四个大质粒。对RtTA1文库BAC克隆进行测序以及从RtTA1基因组中对多糖基因进行PCR扩增，这两者相结合，使得我们鉴定出了五个大的区域和基因簇，以及许多分散在基因组中的单独的潜在多糖生物合成基因。我们观察到明显存在大量可能与脂多糖生物合成相关的基因。所有RtTA1多糖生物合成区域在豌豆根瘤菌蚕豆生物型和/或费氏中华根瘤菌之间都显示出高度保守的同线性。大多数显示出保守顺序的基因也具有较高的序列同一性水平。
