Jeon Jeong-Ryae, Kim Jong-Yeon
Department of Physiology and Obesity-Diabetes Advanced Research Center, Yeungnam University College of Medicine, Daegu, Republic of Korea.
Biol Pharm Bull. 2006 Oct;29(10):2111-5. doi: 10.1248/bpb.29.2111.
The present study examined the anti-obesity effects of pine needle extract (PNE) in 3T3-L1 preadipocytes and in vivo studies. PNE treatment suppressed both glycerol-3-phosphate dehydrogenase activity and expression of peroxisome proliferator-activated receptor (PPAR) gamma in cultured 3T3-L1 adipocytes. To investigate the effect of PNE on obesity in rats fed high-fat diet, four types of diet, which included a normal diet (ND), high-fat diet (HFD), ND+PNE, and HFD+PNE diets, were fed to the rats ad libitum for 6 weeks. The PNE supplement significantly decreased body weight gain and visceral fat mass compared to the HFD group. The total cholesterol, TG, and leptin levels in the plasma were significantly reduced by PNE supplementation compared with those of the HFD group. Histological findings in liver tissue showed that PNE supplementation alleviated steatosis induced by HFD. In conclusion, PNE treatment suppressed differentiation of 3T-L1 adipocytes, in part by down-regulating expression of PPAPgamma mRNA, and reduced adipose tissue mass, hyperlipidemia, and hepatic steatosis in obese rats fed HFD. Therefore, pine needle water extract may be considered for use in therapy to control obesity.
本研究在3T3-L1前脂肪细胞中以及在体内研究中检测了松针提取物（PNE）的抗肥胖作用。PNE处理抑制了培养的3T3-L1脂肪细胞中甘油-3-磷酸脱氢酶的活性以及过氧化物酶体增殖物激活受体（PPAR）γ的表达。为了研究PNE对高脂饮食喂养大鼠肥胖的影响，将四种类型的饮食，即正常饮食（ND）、高脂饮食（HFD）、ND + PNE和HFD + PNE饮食，随意喂养大鼠6周。与HFD组相比，补充PNE显著降低了体重增加和内脏脂肪量。与HFD组相比，补充PNE后血浆中的总胆固醇、甘油三酯和瘦素水平显著降低。肝脏组织的组织学结果表明，补充PNE减轻了HFD诱导的脂肪变性。总之，PNE处理部分通过下调PPAPγ mRNA的表达抑制了3T-L1脂肪细胞的分化，并减少了高脂饮食喂养肥胖大鼠的脂肪组织量、高脂血症和肝脂肪变性。因此，松针水提取物可考虑用于控制肥胖的治疗。
