Bulgakova V G, Korolev P N, Konoshenko G I, Novozhilova T Iu, Polin A N
Mikrobiologiia. 1990 Jul-Aug;59(4):702-4.
The work was aimed at studying the effect of gramicidin S on the intact cells, spheroplasts and membrane specimens of Escherichia coli K12S with the natural resistance to this antibiotic. The resistance was shown to be caused by the barrier properties of the cell wall: the spheroplasts were highly sensitive to the lytic action of gramicidin S. The differences in the sensitivity to gramicidin S of substrate oxidation carried by the membranes of E. coli and Micrococcus luteus, a sensitive organism, were not of crucial significance for the manifestation of the resistance. The resistance was not associated with the decrease of gramicidin S adsorption: the cells were capable of binding large quantities of the antibiotic and remaining viable. Gramicidin S appeared to be attached to the cell walls (most likely, the outer membranes) rather than the cytoplasmic membranes.
该研究旨在探讨短杆菌肽S对具有天然抗药性的大肠杆菌K12S完整细胞、原生质体和膜标本的影响。结果表明，这种抗药性是由细胞壁的屏障特性引起的：原生质体对短杆菌肽S的裂解作用高度敏感。对短杆菌肽S敏感的藤黄微球菌与具有抗药性的大肠杆菌膜上底物氧化敏感性的差异，对于抗药性的表现并非至关重要。抗药性与短杆菌肽S吸附量的减少无关：细胞能够结合大量抗生素并保持存活。短杆菌肽S似乎附着在细胞壁（很可能是外膜）而非细胞质膜上。
