Schwartz Elisabeth F, Schwartz Carlos A, Gómez-Lagunas Froylan, Zamudio Fernando Z, Possani Lourival D
Department of Physiological Sciences, Institute of Biological Sciences, University of Brasilia, Brasilia, DF 70910-900, Brazil.
Toxicon. 2006 Dec 15;48(8):1046-53. doi: 10.1016/j.toxicon.2006.08.009. Epub 2006 Sep 5.
A novel toxin was identified, purified and characterized from the venom of the Mexican scorpion Hadrurus gertschi (abbreviated HgeTx1). It has a molecular mass of 3950 atomic mass units (a.m.u.) and contains 36 amino acids with four disulfide bridges established between Cys1-Cys5, Cys2-Cys6, Cys3-Cys7 and Cys4-Cys8. It blocks reversibly the Shaker B K(+)-channels with a Kd of 52nM. HgeTx1 shares 60%, 45% and 40% sequence identity, respectively, with Heterometrus spinnifer toxin1 (HsTX1), Scorpio maurus K(+)-toxin (maurotoxin) and Pandinus imperator toxin1 (Pi1), all four-disulfide bridged toxins. It is 57-58% identical with the other scorpion K(+)-channel toxins that contain only three disulfide bridges. Sequence comparison, chain length and number of disulfide bridges analysis classify HgeTx1 into subfamily 6 of the alpha-KTx scorpion toxins (systematic name: alpha-KTx 6.14).
从墨西哥蝎子哈德鲁鲁斯·格氏蝎（简称HgeTx1）的毒液中鉴定、纯化并表征了一种新型毒素。它的分子量为3950原子质量单位（a.m.u.），含有36个氨基酸，在Cys1-Cys5、Cys2-Cys6、Cys3-Cys7和Cys4-Cys8之间形成了四个二硫键。它以52nM的解离常数可逆地阻断Shaker B K(+)通道。HgeTx1与异距蝎毒素1（HsTX1）、北非蝎K(+)毒素（蝎毒素）和帝王蝎毒素1（Pi1）（均为四二硫键桥毒素）的序列同一性分别为60%、45%和40%。它与其他仅含三个二硫键的蝎子K(+)通道毒素有57-58%的同一性。序列比较、链长和二硫键数量分析将HgeTx1归类为α-KTx蝎子毒素亚家族6（系统名称：α-KTx 6.14）。
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