Chow L P, Chang K H, Lin J Y
Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, R.O.C.
Biochem Int. 1990 Oct;22(1):95-102.
A highly specific monoclonal antibody (anti-AFP) against alpha-fetoprotein (AFP) was linked to N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) to form conjugates which were purified with a protein A-sepharose CL-4B affinity column. The conjugate, PDP-Anti-AFP was then covalently coupled to the toxic abrin-A chain to synthesize immunotoxins. The immunotoxin, anti-AFP-abrin-A conjugate, which was also purified with a protein A-sepharose CL-4B affinity column, had a molecular weight of 180,000 and had 80% antigen-binding activity that of anti-AFP activity and 92% toxicity of abrin-A chain. The immunotoxin showed selective cytotoxicities toward the AFP secreting human hepatoma cell lines, such HepG2 and Hep3B, but not toward AFP non-secreting human hepatoma cell line, PLC/PRF/5.
一种针对甲胎蛋白（AFP）的高特异性单克隆抗体（抗AFP）与N-琥珀酰亚胺基-3-（2-吡啶基二硫代）丙酸酯（SPDP）相连，形成缀合物，并用蛋白A-琼脂糖CL-4B亲和柱进行纯化。然后将缀合物PDP-抗AFP与毒性相思豆毒素A链共价偶联，以合成免疫毒素。同样用蛋白A-琼脂糖CL-4B亲和柱纯化的免疫毒素抗AFP-相思豆毒素A缀合物，分子量为180,000，具有抗AFP活性的80%抗原结合活性和相思豆毒素A链92%的毒性。该免疫毒素对分泌AFP的人肝癌细胞系，如HepG2和Hep3B显示出选择性细胞毒性，但对不分泌AFP的人肝癌细胞系PLC/PRF/5则无此作用。
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