细胞周期蛋白依赖性激酶8模块在空间上阻止中介体与RNA聚合酶II相互作用。
The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II.
作者信息
Elmlund Hans, Baraznenok Vera, Lindahl Martin, Samuelsen Camilla O, Koeck Philip J B, Holmberg Steen, Hebert Hans, Gustafsson Claes M
机构信息
Department of Biosciences and Nutrition, Karolinska Institutet and School of Technology and Health, Royal Institute of Technology, Novum, SE-141 87 Huddinge, Sweden.
出版信息
Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15788-93. doi: 10.1073/pnas.0607483103. Epub 2006 Oct 16.
Abstract
CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.
摘要
细胞周期蛋白依赖性激酶8（CDK8）与细胞周期蛋白C（CycC）、中介体12（Med12）和中介体13一起，形成一个抑制模块（Cdk8模块），该模块可阻止RNA聚合酶II（pol II）与中介体相互作用。在此，我们报告Cdk8模块阻止pol II相互作用的能力独立于Cdk8依赖性激酶活性。我们使用电子显微镜和单颗粒重建技术来证明Cdk8模块形成一个独特的结构实体，该实体与中介体的头部和中部区域结合，从而在空间上阻碍与pol II的相互作用。
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