Middelhoven Wouter J, van Doesburg Wim
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
Antonie Van Leeuwenhoek. 2007 Feb;91(2):191-6. doi: 10.1007/s10482-006-9103-9.
A slow growing bacterial population able to utilize hexamethylelenetetramine (urotropine) as sole source of carbon, nitrogen and energy was isolated from soil. From this crude enrichment culture two bacteria were isolated and identified as Brevundimonas diminuta and a Phyllobacterium sp. by sequencing of 16S ribosomal DNA. These bacteria also grew on urotropine but at a lower rate than the enrichment culture. Addition of glucose to the latter resulted in growth of some yeasts that overgrew the bacteria. Assimilation of urotropine as sole nitrogen source is very common among yeasts, 46 out of 60 species tested showed this characteristic.
从土壤中分离出一种生长缓慢的细菌群体,该群体能够利用六亚甲基四胺(乌洛托品)作为唯一的碳、氮和能量来源。从这种原始富集培养物中分离出两种细菌,通过对16S核糖体DNA进行测序,鉴定为缺陷短波单胞菌和一种叶杆菌属细菌。这些细菌也能在乌洛托品上生长,但生长速度比富集培养物慢。向后者添加葡萄糖会导致一些酵母生长,这些酵母会过度生长并超过细菌。以乌洛托品作为唯一氮源进行同化在酵母中非常普遍,在所测试的60种酵母中,有46种表现出这种特性。