Poghossian A, Abouzar M H, Amberger F, Mayer D, Han Y, Ingebrandt S, Offenhäusser A, Schöning M J
Aachen University of Applied Sciences (Jülich Campus), Laboratory for Chemical Sensors and Biosensors, Ginsterweg 1, D-52428 Jülich, Germany.
Biosens Bioelectron. 2007 Apr 15;22(9-10):2100-7. doi: 10.1016/j.bios.2006.09.014. Epub 2006 Oct 19.
Field-effect-based capacitive electrolyte-insulator-semiconductor (EIS) sensors have been utilised for the deoxyribonucleic acid (DNA) immobilisation and hybridisation detection as well as for monitoring the layer-by-layer adsorption of polyelectrolytes (anionic poly(sodium 4-styrene sulfonate) (PSS) and cationic poly(allylamine hydrochloride) (PAH)). The EIS sensors with charged macromolecules have been systematically characterised by capacitance-voltage, constant-capacitance, impedance spectroscopy and atomic-force microscopy methods. The effect of the number and polarity of the polyelectrolyte layers on the shift of the capacitance-voltage curves has been investigated. Alternating potential shifts of about 30-90 mV have been observed after the adsorption of each polyanion and polycation layer, respectively. The DNA immobilisation and hybridisation signals were 35-55 and 24-33 mV, respectively. The possible mechanisms for the sensor responses are discussed.
基于场效应的电容式电解质-绝缘体-半导体（EIS）传感器已被用于脱氧核糖核酸（DNA）的固定和杂交检测，以及监测聚电解质（阴离子型聚（4-苯乙烯磺酸钠）（PSS）和阳离子型聚（烯丙胺盐酸盐）（PAH））的逐层吸附。带有带电大分子的EIS传感器已通过电容-电压、恒电容、阻抗谱和原子力显微镜方法进行了系统表征。研究了聚电解质层数和极性对电容-电压曲线偏移的影响。分别吸附每个聚阴离子和聚阳离子层后，观察到约30-90 mV的交变电位偏移。DNA固定和杂交信号分别为35-55 mV和24-33 mV。讨论了传感器响应的可能机制。
