Li Zu-guo, Liu Teng-fei, Xie Wei-bing, Zhou Jun, Yu Li, Ding Yan-qing
Key Laboratory of Functional Proteomics and Transcriptomics of Main Human Diseases of Guangdong Province and Ministry of Education, Department of Pathology, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Oct;26(10):1408-11.
To investigate cyclooxygenase-2 (COX-2) expression of in colorectal carcinoma cell lines and tissues and its clinical implications.
SP immunohistochemistry was used to detect COX-2 protein in SW480 and SW620 cell lines and 50 primary colorectal carcinoma and 50 lymphoma metastasis carcinoma specimens. Real-time PCR was used to detect COX-2 mRNA expression in SW480 and SW620 cell lines.
SW480 and SW620 cell lines both highly expressed COX-2. The positive expression levels of COX-2 increased significantly in lymph node metastatic carcinoma in comparison with primary colorectal carcinoma (P<0.05). The expression COX-2 mRNA in SW620 cell line was higher than that of SW480 cell line, showing a mean expression increment of 2.268 folds in SW620 cell line relative to SW480.
Elevated COX-2 expression may be associated with lymph node metastases of colorectal carcinoma.
研究环氧化酶-2（COX-2）在大肠癌细胞系和组织中的表达及其临床意义。
采用SP免疫组织化学法检测SW480和SW620细胞系以及50例原发性大肠癌和50例淋巴结转移癌标本中的COX-2蛋白。采用实时荧光定量PCR法检测SW480和SW620细胞系中COX-2 mRNA的表达。
SW480和SW620细胞系均高表达COX-2。与原发性大肠癌相比，淋巴结转移癌中COX-2的阳性表达水平显著升高（P<0.05）。SW620细胞系中COX-2 mRNA的表达高于SW480细胞系，相对于SW480细胞系，SW620细胞系的平均表达增加了2.268倍。
COX-2表达升高可能与大肠癌的淋巴结转移有关。
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