McNulty Hyesun P, Byun Jungsoo, Lockwood Samuel F, Jacob Robert F, Mason R Preston
Elucida Research, 100 Cummings Center, Suite 135L, P.O. Box 7100, Beverly, MA 01915-0091, USA.
Biochim Biophys Acta. 2007 Jan;1768(1):167-74. doi: 10.1016/j.bbamem.2006.09.010. Epub 2006 Sep 22.
The biological benefits of certain carotenoids may be due to their potent antioxidant properties attributed to specific physico-chemical interactions with membranes. To test this hypothesis, we measured the effects of various carotenoids on rates of lipid peroxidation and correlated these findings with their membrane interactions, as determined by small angle X-ray diffraction approaches. The effects of the homochiral carotenoids (astaxanthin, zeaxanthin, lutein, beta-carotene, lycopene) on lipid hydroperoxide (LOOH) generation were evaluated in membranes enriched with polyunsaturated fatty acids. Apolar carotenoids, such as lycopene and beta-carotene, disordered the membrane bilayer and showed a potent pro-oxidant effect (>85% increase in LOOH levels) while astaxanthin preserved membrane structure and exhibited significant antioxidant activity (40% decrease in LOOH levels). These findings indicate distinct effects of carotenoids on lipid peroxidation due to membrane structure changes. These contrasting effects of carotenoids on lipid peroxidation may explain differences in their biological activity.
某些类胡萝卜素的生物学益处可能归因于其强大的抗氧化特性，这是由它们与细胞膜的特定物理化学相互作用所决定的。为了验证这一假设，我们测量了各种类胡萝卜素对脂质过氧化速率的影响，并将这些结果与其通过小角X射线衍射方法测定的膜相互作用相关联。在富含多不饱和脂肪酸的膜中评估了手性类胡萝卜素（虾青素、玉米黄质、叶黄素、β-胡萝卜素、番茄红素）对脂质过氧化氢（LOOH）生成的影响。非极性类胡萝卜素，如番茄红素和β-胡萝卜素，会破坏膜双层结构，并表现出强大的促氧化作用（LOOH水平增加>85%），而虾青素则能保持膜结构，并表现出显著的抗氧化活性（LOOH水平降低40%）。这些发现表明，由于膜结构的变化，类胡萝卜素对脂质过氧化有不同的影响。类胡萝卜素对脂质过氧化的这些相反作用可能解释了它们生物学活性的差异。
