Fang Jing, Zhou Qiong, Liu Ling-Zhi, Xia Chang, Hu Xiaowen, Shi Xianglin, Jiang Bing-Hua
The Institute for Nutritional Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Carcinogenesis. 2007 Apr;28(4):858-64. doi: 10.1093/carcin/bgl205. Epub 2006 Oct 27.
Apigenin is a non-toxic dietary flavonoid with anti-tumor properties. We recently showed that apigenin-inhibited hypoxia-inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) expression in human ovarian cancer cells under normoxic condition. However, the effect of apigenin in angiogenesis remains to be elucidated. Angiogenesis is the formation of new blood vessels and is required for tumor growth and metastasis. In this study, we showed that apigenin-inhibited expression of HIF-1 and VEGF in different cancer cells under both normoxic and hypoxic conditions. We demonstrated that apigenin significantly inhibited tumor angiogenesis in vivo, by using both the chicken chorioallantoic membrane and Matrigel plug assays. The inhibition of tumor angiogenesis was associated with the decrease of HIF-1 and VEGF in tumor tissues. Taken together, our results show that apigenin suppresses tumor angiogenesis through HIF-1 and VEGF expression.
芹菜素是一种具有抗肿瘤特性的无毒膳食类黄酮。我们最近发现，芹菜素在常氧条件下可抑制人卵巢癌细胞中缺氧诱导因子-1（HIF-1）和血管内皮生长因子（VEGF）的表达。然而，芹菜素在血管生成中的作用仍有待阐明。血管生成是新血管的形成过程，是肿瘤生长和转移所必需的。在本研究中，我们发现芹菜素在常氧和缺氧条件下均可抑制不同癌细胞中HIF-1和VEGF的表达。我们通过鸡胚绒毛尿囊膜和基质胶栓塞试验证明，芹菜素在体内可显著抑制肿瘤血管生成。肿瘤血管生成的抑制与肿瘤组织中HIF-1和VEGF的减少有关。综上所述，我们的结果表明芹菜素通过HIF-1和VEGF的表达抑制肿瘤血管生成。
