Deng W, Roberts S G E
Faculty of Life Sciences, The Michael Smith Building, The University of Manchester, Oxford Road, Manchester M13 9PT, UK.
Biochem Soc Trans. 2006 Dec;34(Pt 6):1051-3. doi: 10.1042/BST0341051.
The general transcription factor TFIIB (transcription factor IIB) plays a critical role in the assembly of the RNA polymerase II pre-initiation complex. TFIIB can make sequence-specific DNA contacts both upstream and downstream of the TATA box. This has led to the definition of two core promoter BREs (TFIIB-recognition elements), one upstream [BRE(u) (upstream BRE)] and one downstream of TATA box [BRE(d) (downstream BRE)]. TFIIB-BRE(u) and TFIIB-BRE(d) contacts are mediated by two independent DNA-recognition motifs within the core domain of TFIIB. Both the BRE(u) and the BRE(d) modulate the transcriptional potency of a promoter. However, the net effect of the BREs on promoter activity is dependent on the specific blend of elements present within a core promoter.
通用转录因子TFIIB（转录因子IIB）在RNA聚合酶II预起始复合物的组装中起关键作用。TFIIB可在TATA框的上游和下游与序列特异性DNA发生接触。这导致了两种核心启动子BRE（TFIIB识别元件）的定义，一种位于上游[BRE(u)（上游BRE）]，另一种位于TATA框下游[BRE(d)（下游BRE）]。TFIIB与BRE(u)和TFIIB与BRE(d)的接触由TFIIB核心结构域内的两个独立DNA识别基序介导。BRE(u)和BRE(d)均调节启动子的转录能力。然而，BRE对启动子活性的净效应取决于核心启动子中存在的特定元件组合。
