Ibanez Chrystelle, Ito Daisuke, Zawadzka Malgorzata, Jeffery Nick D, Franklin Robin J M
Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.
Glia. 2007 Jan 15;55(2):144-51. doi: 10.1002/glia.20443.
Olfactory ensheathing cells (OECs), the principal glial cells of the peripheral olfactory system, have many phenotypic similarities with Schwann cells of the peripheral nervous system. This makes reliably distinguishing these two cells types difficult, especially following transplantation into areas of injury in the central nervous system. In an attempt to identify markers by which these two cells types can be distinguished, a recent proteomic analysis of fetal OECs and adult Schwann cells identified the actin-binding protein calponin as a potential marker expressed by OECs but not Schwann cells. Since many studies designed with the translational goal of autologous transplantation in mind have used adult OECs, this study examined the expression of calponin by adult OECs, both in vivo within the peripheral olfactory system and in vitro. Calponin colocalized with strongly fibronectin positive fibroblasts in the olfactory mucosa (OM) and meningeal cells in the olfactory bulb (OB) but not with S100beta or neuropeptide-Y positive OECs. In tissue culture, calponin was strongly expressed by fibronectin-expressing fibroblasts from OM, sciatic nerve and skin and by meningeal cells from the OB, but not by p75(NTR)- and S100beta-expressing OECs. These data, supported by Western blotting, indicate that calponin can not be used to distinguish adult OECs and Schwann cells.
嗅鞘细胞(OECs)是外周嗅觉系统的主要神经胶质细胞,与外周神经系统的施万细胞有许多表型相似性。这使得可靠地区分这两种细胞类型变得困难,尤其是在将它们移植到中枢神经系统损伤区域之后。为了确定能够区分这两种细胞类型的标志物,最近一项对胎儿OECs和成年施万细胞的蛋白质组学分析确定肌动蛋白结合蛋白钙调蛋白是一种由OECs而非施万细胞表达的潜在标志物。由于许多旨在自体移植这一转化目标的研究都使用了成年OECs,本研究检测了成年OECs在体内外周嗅觉系统和体外钙调蛋白的表达情况。钙调蛋白在嗅觉黏膜(OM)中与强纤连蛋白阳性成纤维细胞以及嗅球(OB)中的脑膜细胞共定位,但不与S100β或神经肽Y阳性的OECs共定位。在组织培养中,来自OM、坐骨神经和皮肤的表达纤连蛋白的成纤维细胞以及来自OB的脑膜细胞强烈表达钙调蛋白,但表达p75(NTR)和S100β的OECs不表达。这些数据得到蛋白质印迹法的支持,表明钙调蛋白不能用于区分成年OECs和施万细胞。