Stone Daniel, Lieber Andre
Division of Medical Genetics, University of Washington, Seattle, WA 98195, USA.
Curr Opin Mol Ther. 2006 Oct;8(5):423-31.
Since it was first demonstrated that recombinant adenovirus (Ad) vectors could be generated by inserting foreign genes into Ad genomes, they have been extensively used as eukaryotic expression vectors and therapeutic gene delivery vehicles. Originally, Ad vectors were based on the human Ad5 serotype and while most currently used Ad vectors are still based on Ad5, recent research has highlighted a series of problems that limit the efficacy and safety of these vectors. To circumvent the problems associated with Ad5-based vectors, new vectors have utilized various Ads of both human and nonhuman origin. In this review we introduce the recent advances made in the development of non-Ad5-based vectors.
自从首次证明通过将外源基因插入腺病毒（Ad）基因组可以产生重组腺病毒载体以来，它们已被广泛用作真核表达载体和治疗性基因递送工具。最初，腺病毒载体基于人Ad5血清型，虽然目前大多数使用的腺病毒载体仍然基于Ad5，但最近的研究突出了一系列限制这些载体有效性和安全性的问题。为了规避与基于Ad5的载体相关的问题，新的载体利用了各种源自人类和非人类的腺病毒。在这篇综述中，我们介绍了基于非Ad5的载体开发方面的最新进展。
