Kondaurova E M, Bazovkina D V, Kulikov A V, Popova N K
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
Genes Brain Behav. 2006 Nov;5(8):596-601. doi: 10.1111/j.1601-183X.2006.00212.x.
Catalepsy (pronounced motor inhibition) is a natural defensive reaction against predator. Recently, the quantitative trait locus for catalepsy was mapped on mouse chromosome 13 near the 5-HT(1A) serotonin receptor gene. Here, the linkage between catalepsy and the 5-HT(1A) receptor gene was verified using breeding experiment. Selective breeding for high predisposition to catalepsy was started from backcross BC[CBA x (CBA x AKR)] generation between catalepsy-prone (CBA) and catalepsy-resistant (AKR) mouse strains. CBA and AKR strains also differed in the 5-HT(1A) receptor functional activity. A rapid increase of cataleptic percentage from 21.2% in the backcrosses to 71% in the third generation of selective breeding (S3) was shown. The fragment of chromosome 13 including the 5-HT(1A) receptor gene was marked with D13Mit76 microsatellite. Breeding for catalepsy increased the concentration of CBA-derived and decreased the concentration of AKR-derived alleles of microsatellite D13Mit76 in the S1 and S2. All mice of the S9 and S12 were homozygous for CBA-derived allele of D13Mit76 marker. Mice of the S12 showed CBA-like receptor activity. These findings indicate that selective breeding for behavior can involve selection of polymorphic variants of the 5-HT(1A) receptor gene.
僵住症（表现为运动抑制）是一种针对捕食者的自然防御反应。最近，僵住症的数量性状基因座被定位在小鼠13号染色体上靠近5-羟色胺（5-HT）1A受体基因的位置。在此，通过育种实验验证了僵住症与5-HT1A受体基因之间的连锁关系。从易患僵住症的（CBA）和抗僵住症的（AKR）小鼠品系之间的回交BC[CBA×（CBA×AKR）]代开始，对高度易患僵住症进行选择性育种。CBA和AKR品系在5-HT1A受体功能活性方面也存在差异。结果显示，僵住症百分比从回交后代中的21.2%迅速增加到第三代选择性育种（S3）中的71%。包含5-HT1A受体基因的13号染色体片段用D13Mit76微卫星进行了标记。在S1和S2代，针对僵住症的育种增加了微卫星D13Mit76中源自CBA的等位基因浓度，并降低了源自AKR的等位基因浓度。S9和S12的所有小鼠对于D13Mit76标记的源自CBA的等位基因都是纯合的。S12的小鼠表现出类似CBA的受体活性。这些发现表明，针对行为的选择性育种可能涉及对5-HT1A受体基因多态性变体的选择。
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