Ultrastructural localization of glycosaminoglycans in the human mammary gland.

The distribution of glycosaminoglycans (GAGs) in the human breast tissue has been studied at the EM-level by means of the dye Cupromeronic Blue and Scott's critical electrolyte concentration technique. There have been several reproducable localizations: 1. Orthogonally at the main bands of collagen fibrils (probably chondroitin/dermatan sulphate). 2. Large GAGs in areas, almost free of collagen and other fibrils (probably chondroitin sulphate). 3. In the basement membranes of capillaries and glandular epithelium (probably heparan sulphate). 4. Fine GAGs at the surface of the adipocytes (probably heparan sulphate). 5. In the heterogenous granules of mast cells (probably heparin).